Perpustakaan Terpadu

Jeruk nipis (Citrus aurantifolia) merupakan bahan alam yang memiliki potensi dapat menghambat pertumbuhan bakteri. Mencuci dengan sabun cuci tangan cair akan lebih efektif untuk membunuh kuman karena sediaan sabun dalam bentuk cair memiliki sifatunggul seperti lebih ekonomis, higienis, mudah diproduksi, dan mengandung banyak busa. Pengujian sabun cair cuci tangan diharapkan sesuai SNI 2588:2017 pengujian yang dilakukan meliputi ph, bobot jenis, total bahan aktif, bahan yang tidak larut etanol, alkali bebas, asam lemak bebas, serta pengujian daya hambat terhadap bakteri Escherichia coli. Konsentrasi ekstrak jeruk nipis yang digunakan dalam formulasi sabun cair cuci tangan ialah (0ml,10ml, 20ml, dan 30ml) dan pada hasil penelitian formula sediaan sabun cair cuci tangan dari ekstrak Buah jeruk nipis Pengujian mutu sabun cair menunjukkan rentan nilai pH 6,75 sampai 9,47 memenuhi syarat SNI (4-10).Bobot jenis menunjukkan nilai tertinggi 1,042 (F3) memenuhi syarat SNI (1,01 -1,1) dan terendah 0,980 (F2). Total bahan aktif menunjukkan nilai tertinggi 35,85% memenuhi syarat SNI (min 10) dan terendah 5. Bahan yang tidak larut etanol menunjukkan rentan nilai 0,992% – 1,181% tidak memenuhi syarat SNI (maks 0,5). Alkali bebas menunjukkan rentan nilai 1,323% – 2,978% tidak memenuhi syarat SN 2588:2017 (maks 0,5). Asam lemak bebas menunjukkan nilai terendah 0,38 % (F2) memenuhi syarat SNI (maks 1) dan nilai tertinggi 10,50% (F1). Untuk uji organoleptik menunjukkan semua formula memenuhi SNI yaitu bentuk cairan homogen, bau khas dan warna khas yaitu kuning pucat (F1,F2,F3) dan bau jeruk nipis. Berdasarkan 4 formula yang telah dibuat maka dapat ditarik kesimpulan bahwa 4 formulasi yang dibuat tidak memenuhi SNI.Untuk pengujian mikrobiologi didapatkan zona hambat 7mm (F1) yang artinya daya hambat cukup, 12,25 mm pada (F2) yang artinya daya hambat kuat, 12 mm (F3) yan artinya daya hambat kuat dan dapat disimpulkan bahwa sediaan sabun caair cuci tangan ekstrak jeruk nipis efektif terhadap bakteri Escherichia coli. Kata kunci : jeruk nipis, sabun cair cuci tangan,daya hambat bakteri, Escherchia coli

Lime (Citrus aurantifolia) is a natural ingredient that has the potential to inhibit bacterial growth. Washing with liquid hand washing soap will be more effective in killing germs because soap preparations in liquid form have superior properties such as being more economical, hygienic, easy to produce, and containing a lot of foam. Testing of liquid handwashing soap is expected to be in accordance with SNI 2588:2017. The tests carried out include pH, specific gravity, total active ingredients, ethanol-insoluble ingredients, free alkali, free fatty acids, as well as testing the inhibition of Escherichia coli bacteria. The concentration of lime extract used in the formulation of liquid hand washing soap is 0 ml, 10 ml, 20 ml, and 30 ml, and in the results of the study, the formula for liquid hand washing soap was made from lime fruit extract. 9.47 meets SNI requirements (4–10). The specific gravity shows the highest value is 1.042 (F3), which meets SNI requirements (1.01-1.1), and the lowest is 0.980 (F2). Total active ingredients show the highest value of 35.85% fulfilling SNI requirements (min. 10) and the lowest value of 5. Materials that are insoluble in ethanol show a susceptibility value of 0.992%–1.181% not meeting SNI requirements (max 0.5). Free alkaline shows a vulnerable value of 1.323% – 2.978% does not meet the requirements of SN 2588:2017 (max 0.5). Free fatty acids show the lowest value of 0.38% (F2) fulfilling the SNI requirements (max 1), and the highest value of 10.50% (F1). The organoleptic test showed that all formulas met SNI, namely homogeneous liquid form, characteristic odor, and characteristic color, namely pale yellow (F1, F2, F3) and the smell of lime. Based on the 4 formulas that have been made, it can be concluded that the 4 formulations that were made did not meet SNI. For microbiological testing, the inhibition zone was 7mm (F1), which means the inhibition was sufficient, 12.25 mm (F2), which means the inhibition was strong; and 12 mm (F3), which means strong inhibition, and it can be concluded that lime extract liquid handwashing soap is effective against Escherichia coli bacteria. Keywords: lime, liquid handwashing soap, inhibition of bacteria, Escherichia coli