Perpustakaan Terpadu

Fenol merupakan metabolit sekunder terbesar dalam tumbuhan. Senyawa fenol banyak terdapat dalam tumbuhan seperti sayuran dan buah-buahan. Salah satu buah yang mengandung senyawa fenol yaitu buah salak, dimana daging buah dan kulitnya diketahui memiliki aktivitas antioksidan. Beberapa penelitian mengenai biji buah salak menyatakan bahwa biji buah salak memliki aktivitas sitotoksik, antibakteri dan antioksidan. Namun masih sedikit penelitian mengenai analisis kandungan senyawa fitokimia terhadap biji salak untuk varietas Bali. Penelitian ini bertujuan untuk menentukan kadar total fenol dalam berbagai varian biji buah salak varietas Bali dari perkebunan salak di Desa Sibetan Kabupaten Karangasem. Penentuan kadar total fenol dilakukan pada ekstrak etanol biji buah salak varietas Bali dengan metode Spektrofotometri UV-Vis pada panjang gelombang 765 nm menggunakan standar asam galat. Fenol ditentukan dengan metode Total Phenolic Content berdasarkan prinsip peningkatan intensitas warna yang bereaksi dengan reagen Folin-Ciocalteu, dan diukur kadar total fenol secara Spektrofotometri UV-Vis. Berdasarkan hasil penelitian pada 5 varian biji buah salak Bali (Salacca zalanca var. ambonensis), dapat disimpulkan bahwa kadar total fenol tertinggi terdapat pada ekstrak etanol biji salak gula pasir yaitu sebesar 12,8763 mg GAE/g Ekstrak atau 1,2876% b/b dan kadar terendah terdapat pada ekstrak etanol biji salak nangka yaitu sebesar 6,8932 mg GAE/g Ekstrak atau 0,6893% b/b.

Phenols are the largest secondary metabolite in plants. Phenol compounds are widely found in plants such as vegetables and fruits. One of the fruits that contain phenolic compounds is snake fruit, where the flesh and skin are known to have activity of antioxidant. Several studies on snake fruit seeds state that snake fruit seeds have cytotoxic, antibacterial and antioxidant activities. However, there is still little research on the analysis of the content of phytochemical compounds on the seeds of snake fruit varieties Bali. The aims of this research were to determine the total phenolic content in some variants of the seeds of snake fruit varieties Bali from the snake fruit plantation in Sibetan Village, Regency of Karangasem. Determination of total phenolic content was carried out on the ethanolic extract of snake fruit seeds varieties Bali with UV-Vis Spectrophotometry method at a wavelength of 765 nm with gallic acid standards. Phenol was determined by the Total Phenolic Content method based on the principle of increasing color intensity by reacting with Folin-Ciocalteu reagent, and the total phenol content was measured by UV-Vis spectrophotometry. Based on the results of research on 5 variants of the seeds of the Balinese snake fruit (Salacca zalanca var. ambonensis), it can be concluded that the highest total phenol content are in the ethanol extract of sugar snake fruit seeds which is 12,8763 mg GAE/g Extract or 1,2876% b/b and the lowest content is in the ethanol extract of jackfruit snake fruit seeds which is 6,8932 mg GAE/g Extract or 0,6893% b/b.