ABSTRACT
Background: Benzo[a]pyrene is one of the external factors that reduce immunity. The disease arises by the accumulation of benzo[a]pyrene in DNA, which forms benzo[a]pyrene and aryl hydrocarbon receptor (AhR bonds). The bond of benzo[a] pyrene and AhR could affect the differentiation of immune cells then induce an immunosuppressive effect. Objective: This research aims to identify the bioactivity of benzo[a]pyrene toward the expression of CD4+ tumor necrosis factor (TNF)α, CD8+ IFNŸ, and CD4+ interleukin (IL)1+ in mice treated with a paramyxovirus vaccine. Materials and Methods: This research was conducted using a split-plot design. The bioactivity analysis of benzo[a]pyrene toward immune cells expression in mice (Mus musculus L) was analyzed by administration of benzo[a]pyrene at 20 mg/kg BW through intramuscular injection 2 times/week for 4 weeks. The expression of CD4+ TNF-α+, CD8+ IFN-Ÿ+, and CD4+IL-1+ was analyzed using flow cytometry. Results: Group with vaccine and benzo[a]pyrene exposure at week 4 showed that TNFα was repressed while IFNŸ was elevated. Furthermore, the modest regulatory function of CD4+ was activated. Conclusion: The exposure of benzo[a]pyrene to the mice (M. musculus L) with dose 20 mg/kg BW for 2 times/weeks exhibited the immune cell repression on CD4+TNFα+ and immune cell activation on CD8+IFNŸ+, while CD4+IL-1+ at week 4.